A small drop in oxygen content has big negative effects on cod

Hypoxia in Danish waters has been widespread in late summer and autumn months and has become increasingly severe in recent years. But do more subtle reductions in oxygen levels affect fish, and to what extent? New surprising results from DTU Aqua points to that even a small reduction in oxygen is having significant consequences.

Iltforsøg i DTU Aquas Fiskestald, foto Sune Riis Sørensen
PhD student Magnus Højen Husen feeds the cod, which swim in tanks in the experimental facility for fish biology at DTU Aqua in Lyngby. Photo by Sune Riis Sørensen.

"We have investigated the effect of an oxygen level, which is at a level that cannot even be defined as oxygen depletion, but is simply called 'low oxygen content' in the annual reports from Aarhus University. This corresponds to 4-6mg oxygen/L. The effect on the cod was significantly more negative than we had actually expected," says Magnus Husen and elaborates:

"The fish that had been at this oxygen level for five weeks had grown significantly less, were thinner and had a smaller liver in relation to their body size than normal. The liver is the cod's 'food package', and with a small liver the fish have less energy available. Their swimming ability was also affected''.

A call for more attention on oxygen depletion

The cod, on the other hand, tried to compensate for the poorer oxygen conditions by up-regulating the amount of red blood cells and the concentration of hemoglobin, which transports oxygen in the blood around the body. According to the researchers, they turned into different cod. Which the researchers could also see in the behavior of the fish:

''Our observations also show that the cod here are less active and react less to external influences. In short, they seem duller, and one can fear that they are therefore also more vulnerable to external threats, for example being hunted and caught by predators," says Magnus Husen.

All in all, the preliminary results from Magnus Husen's experiments show that moderate or severe oxygen depletion does not need to occur before fish such as cod become measurably and chronically affected – the alarm bells should ring as soon as we see a minor reduction of oxygen in the sea, which is not even defined as oxygen depletion. 

Demanding laboratory experiments

The growth experiments with reduced oxygen and cod have been carried out by Magnus Husen and Jane Behrens in DTU Aqua's experimental facility for fish biology in Lyngby over six weeks. Cod from Øresund were divided into two groups, where one group of fish was in fully oxygenated water, while the other group was in water with 50 percent oxygen saturation. See more about the experiments in the fact box.

It is no easy endeavor. The actual set-up with wild cod, which you have to make sure thrive and eat and do not get sick, alongside the fact that you have to maintain a stable oxygen level in many tanks at a time, requires very special facilities and solid knowledge and experience.

DTU Aqua's experimental facility for fish biology in Lyngby is a leader in this type of experiment with wild fish, explains Jane Behrens:

’’Firstly, the facility has a large capacity, which means that we can have many fish swimming in several tanks and over longer periods. We can also control the temperature, salinity and oxygen content of the water and monitor these parameters online, which is why we can do many types of experiments and with different species of fish.” 

"And then our sister facility, which works with fish diseases, has several vets in their group, so we can always quickly get advice and help if some fish show signs of disease," adds Jane Behrens.

Next step – Åland

The study of the effects of reduced oxygen on cod in the experimental facility is just the first step in Magnus Husen's Ph.D. about the impacts of different pressure factors on cod:

"We know that increased levels of hypoxia affect fish, but if we can now demonstrate how just a little reduction in oxygen affects a commercially important species like the cod, then we can better understand some of the factors that have contributed to the miserable state of cod now," says Magnus Husen.

The next steps in the PhD will be to look at some other factors that may also affect the cod's growth and success, such as food quality, quantity and diversity. This spring, Magnus Husen will analyze the stomach contents of the 'super cod' found around Åland in Sweden:

"We will look at how their diet differs from the cod we know here at home," says Magnus Husen.

Up in the Åland Sea, just before the entrance to the northernmost part of the Baltic Sea, the Bothnian Sea and bay, the cod is distinguished by its large size - they grow much larger than their relatives in the more eastern, central and western Baltic Sea.

The Åland cod has good oxygen conditions, and over time there has also only been a small, local fishery for them. But otherwise, little is known about these cod far to the north.

Oxygen and cold water may not be the whole explanation

In connection with the MERTOR project, which Magnus Husen's Ph.D. is part of, both PhD student and supervisor Jane Behrens will go to Åland in northern Sweden in April to study the Åland cod.  
 
"Overall, I am very excited to, together with Magnus, delve deeper into understanding why these cod on Åland are doing so well. I suspect that it is not only the better oxygen conditions and the colder water up there that is the whole explanation," says Jane Behrens and elaborates:
 
"Perhaps part of the explanation also lies in the food they have available, and here I have a feeling that a small circular animal - saduria, also called Baltic crayfish - might play a role." 

In the Åland Sea, the water has lower salinity than the sea around Denmark, and also than in the Baltic Sea - is this also something the researchers want to look at?
 
"Yes, that is absolutely true - the salinity in the Åland Sea is significantly lower than both in the central Baltic Sea and in our internal Danish waters. However, it is not a parameter that we have to look at directly," says Jane Behrens and continues:
 
"But it is believed that this means that the cod in the Åland Sea cannot spawn locally, but must make spawning migrations to other places. However, it is still unclear where they swim to spawn.”
 

A broader insight

The MERTOR project and Magnus Husen's Ph.d. focuse on our national fish, the cod. But can the knowledge and experience gained from the experiments with cod be transferred to other species?
"Yes, you can," says Magnus Husen and explains:

"The set-up we have developed, and the learning from the experiments we are taking with us, can well be transferred to other species. However, this requires a good insight into the biology of the species and how they handle captivity. And then, of course, a facility that has the same capacity and modernity as here at DTU." 

Back to the experiment in the experimental facility for fish biology at DTU Aqua in Lyngby, there are still many exciting samples waiting to be analyzed. Among other things, blood tests, which will be taken with Magnus to Aarhus University, where they have the right equipment to look at the blood's ability to bind oxygen. 

In addition, the test fish's gills, pituitary gland, muscle tissue and otoliths must be examined to fully appreciate what a small reduction in oxygen means for our fish.

 

The experiment

A group of fish was in fully oxygenated water. The second group in water with 50 percent oxygen saturation, which corresponds to just under 5mg/L at the temperature and salinity at which the experiments were carried out.

In this group, the first week was spent lowering the oxygen level slowly from full oxygen saturation to 50 percent oxygen saturation to ensure that the fish had time to adjust to this.

All fish were fed daily with as much sandeel as they wanted to eat, and it was recorded how much they ate. Before the experiment started, all cod had been measured and weighed and marked with a so-called PIT tag, so that the researchers could tell them apart and thus know how much each individual grew.

In addition, the temperature and salinity of the water were kept stable at the cod's natural levels. Before the experiment started, the fish were left to acclimate for 2-4 weeks after they were caught, so they could get used to being in captivity.

 

Oxygen depletion

In the annual assessments of the oxygen conditions in the waters around Denmark, which Aarhus University publishes every year , the reduced oxygen levels are divided into three categories: low oxygen content (the mildest degree of reduced oxygen), moderate oxygen loss and severe oxygen loss. Low oxygen content is calculated to be 4-6 mg oxygen/L.

Learn more about oxygen depletion and pressure on life in the seas around Denmark in the DTU Aqua-report "Development in the state of the seabed in the seas around Denmark: Analyzes to support the status of the marine strategy's descriptor 6"

The project

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Jane Behrens

Jane Behrens Senior Researcher National Institute of Aquatic Resources Mobile: +45 23296863